Novel approach to quantitative detection of specific rRNA in a microbial community, using catalytic DNA.

Using Catalytic DNA of Specific rRNA in a Microbial Community, Novel Approach to Quantitative Detection

Rumen Microbial Community of Saanen Goats Adapted to a High-Fiber Diet in the Northeast of Iran

Swiss Saanen goat is a widespread breed frequently found in commercial herds across the world. The present study aimed to identify the rumen microbial community of exotic Saanen goats adapted to a fibrous diet using barcoded pyrosequencing. Rumen content samples were collected from the four animals via a stomach tube after the morning graze and freeze-dried for DNA extraction. Bacterial and arc...

A study of rumen microbial community of Baluchi lambs fed a high concentrate diet containing conventional ingredients

Baluchi sheep is the dominant fat-tail breed in Iran. We studied the microbial communities in the rumen of four Baluchi lambs fed a high concentrate conventional diet. Using DNA extracted from the rumen samples, we found the partial ribosomal rRNA of bacterial and archaeal were amplified by polymerase chain reaction (PCR). The amplicons were sequenced using 454 Titanium pyrosequencing and the d...

A study of rumen microbial community of Baluchi lambs fed a high concentrate diet containing conventional ingredients

Baluchi sheep is the dominant fat-tail breed in Iran. We studied the microbial communities in the rumen of four Baluchi lambs fed a high concentrate conventional diet. Using DNA extracted from the rumen samples, we found the partial ribosomal rRNA of bacterial and archaeal were amplified by polymerase chain reaction (PCR). The amplicons were sequenced using 454 Titanium pyrosequencing and the d...

Simultaneous Assessment of Soil Microbial Community Structure and Function through Analysis of the Meta-Transcriptome

BACKGROUND Soil ecosystems harbor the most complex prokaryotic and eukaryotic microbial communities on Earth. Experimental approaches studying these systems usually focus on either the soil community's taxonomic structure or its functional characteristics. Many methods target DNA as marker molecule and use PCR for amplification. METHODOLOGY/PRINCIPAL FINDINGS Here we apply an RNA-centered met...